Recombinant SIRT3 antibody

Catalog No. ABIN7938489

The Rabbit Monoclonal anti-SIRT3 antibody (Clone 2P6) (ABIN7938489) specifically detects SIRT3 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant SIRT3 antibody (ABIN7938489)

Target: SIRT3 (Sirtuin 3 (SIRT3))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SIRT3 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 2P6

Product Details anti-SIRT3 Antibody

Purpose: SIRT3 antibody (rAb), 100 μl (Clone 2P6)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against the SIRT3 fusion protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:150000 dilution

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS, pH 7.3, with 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for SIRT3

Target: SIRT3 (Sirtuin 3 (SIRT3))

Alternative Name: SIRT3

Background:

Background_Plain: Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive, and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.

Aliases: SIRT-3, Sirtuin 3, Sir1-Like 3, EC 3.5.1

Molecular Weight: 44 kDa