OGFOD3 antibody (AA 67-311)

Catalog No. ABIN7964811

The Rabbit Polyclonal anti-OGFOD3 antibody (ABIN7964811) specifically detects OGFOD3 in FACS, WB, ELISA, IHC, ICC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for OGFOD3 antibody (AA 67-311) (ABIN7964811)

Target: OGFOD3 (2-Oxoglutarate and Iron-Dependent Oxygenase Domain Containing 3 (OGFOD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OGFOD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-OGFOD3 Antibody

Binding Specificity: AA 67-311

Purpose: Anti-OGFOD3 Antibody

Predicted Reactivity: Human OGFOD3 shares 92.2%,90.6% amino acid (aa) sequence identity with mouse,rat OGFOD3,respectively.

Characteristics: Anti-OGFOD3 Antibody. Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human OGFOD3 recombinant protein (Position: D67-H311). Human OGFOD3 shares 92.2% and 90.6% amino acid (aa) sequence identity with mouse and rat OGFOD3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for OGFOD3

Target: OGFOD3 (2-Oxoglutarate and Iron-Dependent Oxygenase Domain Containing 3 (OGFOD3))

Alternative Name: OGFOD3

Background:

Background: OGFOD3 (2-oxoglutarate and iron-dependent oxygenase domain-containing protein 3) is a protein involved in the hydroxylation of transfer RNA (tRNA), specifically targeting tRNA-hypoxanthine modification. This process is crucial for maintaining accurate translation and protein synthesis in cells. OGFOD3 belongs to the family of 2-oxoglutarate (2OG)-dependent oxygenases, which require iron and 2OG as cofactors to catalyze various oxidative reactions. Its role highlights the importance of tRNA modifications in cellular physiology, potentially influencing gene expression and protein function regulation.

Gene Full Name: 2-oxoglutarate and iron dependent oxygenase domain containing 3

Molecular Weight: 36 kDa

Gene ID: 79701

UniProt: Q6PK18