ACTN2 antibody (AA 567-696)

Catalog No. ABIN7965714

The Rabbit Polyclonal anti-ACTN2 antibody (ABIN7965714) specifically detects ACTN2 in WB, IHC, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ACTN2 antibody (AA 567-696) (ABIN7965714)

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-ACTN2 Antibody

Binding Specificity: AA 567-696

Purpose: Anti-ACTN2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ACTN2 Antibody. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ACTN2 recombinant protein (Position: E567-E696).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ACTN2

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Alternative Name: ACTN2

Background:

Background: Alpha-actinin 2 is a protein which in humans is encoded by the ACTN2 gene. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: actinin alpha 2

Molecular Weight: 104 kDa

Gene ID: 88

UniProt: P35609

Pathways: Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Negative Regulation of Transporter Activity