SUB1 antibody (AA 62-127)

Catalog No. ABIN7965813

The Mouse Monoclonal anti-SUB1 antibody (Clone 8D9D1) (ABIN7965813) specifically detects SUB1 in WB, IHC, FACS and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SUB1 antibody (AA 62-127) (ABIN7965813)

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SUB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Carrier-free

Clone: 8D9D1

Product Details anti-SUB1 Antibody

Binding Specificity: AA 62-127

Purpose: Anti-PC4/SUB1 Antibody (monoclonal, 8D9D1)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PC4/SUB1 Antibody.(monoclonal, 8D9D1)-3. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PC4/SUB1 recombinant protein (Position: N62-L127).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SUB1

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Alternative Name: SUB1

Background:

Background: Activated RNA polymerase II transcriptional coactivator p15, also known as positive cofactor 4 (PC4) or SUB1 homolog, is a protein that in humans is encoded by the SUB1 gene. This gene is mapped to 5p13.3. The transcriptional cofactor PC4 is an ancient single-strand DNA (ssDNA)-binding protein that has a homologue in bacteriophage T5 where it is likely the elusive replicative ssDNA-binding protein. The recombinant PC4 is shown to function identically to the native protein through its interaction with TAFs.

Gene Full Name: SUB1 regulator of transcription

Molecular Weight: 19 kDa

Gene ID: 10923

UniProt: P53999