ADAM17 antibody (AA 215-449) (Fluoro647)

Catalog No. ABIN7966285

This Rabbit Polyclonal antibody specifically detects ADAM17 in FACS. It exhibits reactivity toward Rat and Mouse.

Quick Overview for ADAM17 antibody (AA 215-449) (Fluoro647) (ABIN7966285)

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM17 antibody is conjugated to Fluoro647

Application: Flow Cytometry (FACS)

Product Details anti-ADAM17 Antibody (Fluoro647)

Binding Specificity: AA 215-449

Purpose: Anti-Adam17 Antibody Fluoro647 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Adam17 recombinant protein (Position: R215-M449).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for ADAM17

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name: Adam17

Background:

Background: ADAM17(ADAM metallopeptidase domain 17), also called TACE (tumor necrosis factor-α-converting enzyme), is a 70- kDa enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases. Expression studies showed that the encoded protein cleaves precursor tumor necrosis factor-alpha to its mature form. Northern blot analysis revealed that the gene was expressed as a 5-kb mRNA in all tissues examined. ADAM17 is understood to be involved in the processing of tumor necrosis factor alpha (TNF-α) at the surface of the cell, and from within theintracellular membranes of the trans-Golgi network. This process, which is also known as 'shedding', involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-α), and is of known physiological importance. ADAM17 was the first 'sheddase' to be identified, and is also understood to play a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules,receptors, ligands, and enzymes.

Gene Full Name: a disintegrin and metallopeptidase domain 17

Gene ID: 11491

UniProt: Q9Z0F8

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus