CDC25C antibody (pThr48)

Catalog No. ABIN746993

The Rabbit Polyclonal anti-CDC25C antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect CDC25C in samples from Human, Mouse, Rat and Chicken. There are 2+ publications available.

Quick Overview for CDC25C antibody (pThr48) (ABIN746993)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivity: Human, Mouse, Rat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CDC25C Antibody

Binding Specificity: pThr48

Cross-Reactivity: Chicken, Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Thr48

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CDC25C antibody (ABIN746993)

Yin, Jiang, Peng, Cui, Zhou, He, Zuo, Ouyang, Fan, Fang: "The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum." in: Oncotarget, Vol. 7, Issue 24, pp. 35592-35606, (2016) (PubMed).

Guo, Cui, Peng, Fang, Zuo, Deng, Wang, Wu, Chen, Deng: "Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney." in: Oncotarget, Vol. 6, Issue 34, pp. 35964-77, (2015) (PubMed).

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: cdc25C

Background:

Synonyms: CDC25, PPP1R60, M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C

Background: Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.

Gene ID: 995

UniProt: P30307

Pathways: Cell Division Cycle, M Phase