CDK2 antibody (pThr160)

Catalog No. ABIN747008

The Rabbit Polyclonal anti-CDK2 antibody has been validated for WB, IHC (p) and IF (p). It is suitable to detect CDK2 in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for CDK2 antibody (pThr160) (ABIN747008)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CDK2 Antibody

Binding Specificity: pThr160

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CDK2 around the phosphorylation site of Thr160 [TY(p-T)HE]

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

References for anti-CDK2 antibody (ABIN747008)

Mlcochova, Sutherland, Watters, Bertoli, de Bruin, Rehwinkel, Neil, Lenzi, Kim, Khwaja, Gage, Georgiou, Chittka, Yona, Noursadeghi, Towers, Gupta: "A G1-like state allows HIV-1 to bypass SAMHD1 restriction in macrophages." in: The EMBO journal, Vol. 36, Issue 5, pp. 604-616, (2017) (PubMed).

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: Cdk2

Background:

Synonyms: p-CDH2 Thr160, CDH2 Thr160, CDH2 T160,Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDK2_HUMAN.

Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq, Jul 2008].

Gene ID: 1017

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA