APEX2 antibody (AA 102-210) (Biotin)

Catalog No. ABIN7969257

This Rabbit Polyclonal antibody specifically detects APEX2 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for APEX2 antibody (AA 102-210) (Biotin) (ABIN7969257)

Target: APEX2 (APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-APEX2 Antibody (Biotin)

Binding Specificity: AA 102-210

Purpose: Anti-APEX2 Antibody Biotin Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human APEX2 shares 91.7% amino acid (aa) sequence identity with mouse APEX2.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human APEX2 recombinant protein (Position: L102-A210). Human APEX2 shares 91.7% amino acid (aa) sequence identity with mouse APEX2.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for APEX2

Target: APEX2 (APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2))

Alternative Name: APEX2

Background:

Background: APEX2, also called apurinic/apyrimidinic endonuclease like-2, is a member of the apurinic/apyrimidinic (AP) family of endonucleases that initiate the repair of AP sites formed by spontaneous hydrolysis of the N-glycosylic bond, mutagen-induced base release, or damaged-base excision by a DNA repair glycosylase. RT-PCR detected APEX2 expression in HeLa cells, Jurkat cells, and human kidney, brain and fetal brain tissue. The APEX2 gene is mapped to chromosome Xp11.21. APEX2 participates in both nuclear and mitochondrial base excision repair (BER) and it can play a role in processing 3-prime-damaged termini or 3-prime-mismatched nucleotides. Additionally, APEX2 displayed weaker AP site-specific and 3-prime nuclease activities compared to APEX1.

Gene Full Name: apurinic/apyrimidinic endodeoxyribonuclease 2

Gene ID: 27301

UniProt: Q9UBZ4