APEX2 antibody (AA 1-500)

Catalog No. ABIN7969278

This Rabbit Polyclonal antibody specifically detects APEX2 in WB, FACS and ELISA. It exhibits reactivity toward Human.

Quick Overview for APEX2 antibody (AA 1-500) (ABIN7969278)

Target: APEX2 (APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Grade: Carrier-free

Product Details anti-APEX2 Antibody

Binding Specificity: AA 1-500

Purpose: Anti-APEX2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-APEX2 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human APEX2 recombinant protein (Position: M1-G500).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for APEX2

Target: APEX2 (APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2))

Alternative Name: APEX2

Background:

Background: APEX2, also called apurinic/apyrimidinic endonuclease like-2, is a member of the apurinic/apyrimidinic (AP) family of endonucleases that initiate the repair of AP sites formed by spontaneous hydrolysis of the N-glycosylic bond, mutagen-induced base release, or damaged-base excision by a DNA repair glycosylase. RT-PCR detected APEX2 expression in HeLa cells, Jurkat cells, and human kidney, brain and fetal brain tissue. The APEX2 gene is mapped to chromosome Xp11.21. APEX2 participates in both nuclear and mitochondrial base excision repair (BER) and it can play a role in processing 3-prime-damaged termini or 3-prime-mismatched nucleotides. Additionally, APEX2 displayed weaker AP site-specific and 3-prime nuclease activities compared to APEX1.

Gene Full Name: apurinic/apyrimidinic endodeoxyribonuclease 2

Molecular Weight: 57 kDa

Gene ID: 27301

UniProt: Q9UBZ4