APOE antibody (AA 19-317)

Catalog No. ABIN7969443

This Rabbit Polyclonal antibody specifically detects APOE in WB, IHC, ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for APOE antibody (AA 19-317) (ABIN7969443)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-APOE Antibody

Binding Specificity: AA 19-317

Purpose: Anti-Apolipoprotein E/APOE Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Apolipoprotein E shares 73%,72% amino acid (aa) sequence identity with mouse,rat Apolipoprotein E,respectively.

Characteristics: Anti-Apolipoprotein E/APOE Antibody. Tested in ELISA, Flow Cytometry, IHC, IF, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Apolipoprotein E recombinant protein (Position: K19-H317). Human Apolipoprotein E shares 73% and 72% amino acid (aa) sequence identity with mouse and rat Apolipoprotein E, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunofluorescence, 5 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells ELISA (Cap), 1-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: APOE

Background:

Background: APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.

Gene Full Name: apolipoprotein E

Sequence Similarities: Belongs to the apolipoprotein A1/A4/E family.

Molecular Weight: 36 kDa

Gene ID: 348

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism