ALOX5 antibody (C-Term)

Catalog No. ABIN7969861

The Rabbit Polyclonal anti-ALOX5 antibody is suitable to detect ALOX5 in samples from Human. It has been validated for WB and IHC.

Quick Overview for ALOX5 antibody (C-Term) (ABIN7969861)

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-ALOX5 Antibody

Binding Specificity: C-Term

Purpose: Anti-5 Lipoxygenase/ALOX5 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat,mouse sequences by two amino acids.

Characteristics: Anti-5 Lipoxygenase/ALOX5 Antibody. Tested in IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX5, different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Western blot, 0.1-0.5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: ALOX5

Background:

Background: ALOX5 (ARACHIDONATE 5-LIPOXYGENASE), also known as LOG5 or 5-LO (5-LIPOXYGENASE), is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes which also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases. The enzyme 5-lipoxygenase catalyzes 2 reactions in the formation of leukotrienes. The ALOX5 gene is mapped to chromosome 10q11.21 based on an alignment of the ALOX5 sequence with the genomic sequence. Human 5-LO contains 3 nuclear localization sequences (NLSs) and a phosphorylation site involved in nuclear localization. Compared with age-matched 5-LO competent mice, the 5-LO knockout mice developed less right heart hypertrophy. Pharmacologic inhibition or ALOX5 gene disruption resulted in a significant decrease of beta-amyloid production and gamma-secretase levels. ALOX5 activity is short-lived, apparently in part because of an intrinsic instability of the enzyme.

Gene Full Name: arachidonate 5-lipoxygenase

Sequence Similarities: Belongs to the lipoxygenase family.

Molecular Weight: 78 kDa

Gene ID: 240

UniProt: P09917