Aryl Hydrocarbon Receptor antibody (Middle Region)

Catalog No. ABIN7970191

The Rabbit Polyclonal anti-Aryl Hydrocarbon Receptor antibody is suitable to detect Aryl Hydrocarbon Receptor in samples from Mouse and Rat. It has been validated for WB.

Quick Overview for Aryl Hydrocarbon Receptor antibody (Middle Region) (ABIN7970191)

Target: Aryl Hydrocarbon Receptor (AHR)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aryl Hydrocarbon Receptor antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-Aryl Hydrocarbon Receptor Antibody

Binding Specificity: Middle Region

Purpose: Anti-Aryl hydrocarbon Receptor/AHR Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related human sequence by five amino acids,and from the related rat sequence by one amino acid.

Characteristics: Anti-Aryl hydrocarbon Receptor/AHR Antibody. Tested in WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse AHR, different from the related human sequence by five amino acids, and from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Aryl Hydrocarbon Receptor

Target: Aryl Hydrocarbon Receptor (AHR)

Alternative Name: Ahr

Background:

Background: AHR (aryl hydrocarbon receptor), also called bHLHe76, is a member of the family of basic helix-loop-helix transcription factors. AhR is a cytosolic transcription factor that is normally inactive, bound to several co-chaperones. The AHR gene is mapped on 7p21.1. Estrogenic actions of AHR agonists were detected in wildtype ovariectomized mouse uteri, but were absent in Ahr -/- or Er-alpha -/- ovariectomized mice. Complex assembly and ubiquitin ligase activity of CUL4B (AHR) in vitro and in vivo are dependent on the AHR ligand. In the CUL4B (AHR) complex, ligand-activated AHR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Cd4-positive cells from mice lacking Ahr developed Th17 responses but failed to produce Il22 and did not show enhanced Th17 development. Activation of Ahr during induction of EAE accelerated disease onset and increased pathology in wildtype mice, but not in Ahr -/- mice. The TDO-AHR pathway is active in human brain tumors and is associated with malignant progression and poor survival. Ahr activity within ROR-gamma-t-positive ILC could be induced by dietary ligands such as those contained in vegetables of the family Brassicaceae.

Gene Full Name: aryl-hydrocarbon receptor

Sequence Similarities: Contains 1 bHLH (basic helix-loop-helix) domain.

Molecular Weight: 96 kDa

Gene ID: 11622

UniProt: P30561

Pathways: Regulation of Cell Size