Aurora Kinase B antibody (N-Term)

Catalog No. ABIN7971580

The Rabbit Polyclonal anti-Aurora Kinase B antibody is suitable to detect Aurora Kinase B in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for Aurora Kinase B antibody (N-Term) (ABIN7971580)

Target: Aurora Kinase B (AURKB)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-Aurora Kinase B Antibody

Binding Specificity: N-Term

Purpose: Anti-Aurora B/AURKB Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Aurora B/AURKB Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse Aurora B, different from the related rat sequence by two anino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: AURKB

Background:

Background: AURKB (aurora kinase B) also called Aik2, AIM-1, ARK2, AurB, STK12, IPL1, PPP1R48 or STK5, localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. Cell cycle and Northern blot analyses showed that STK12 is expressed in the S phase and persistently thereafter. Western blot analysis indicated that STK12 is localized in the midbodies during anaphase. Northern blot analysis detected strong expression of a 1.5-kb STK12 transcript in thymus, with weaker expression in small intestine, testis, colon, spleen, and brain. The AURKB gene is mapped on 17p13.1. Examination of the role of both kinases in the phosphorylation of CENPA revealed that the reaction is mediated sequentially by AURKA and AURKB in early mitosis. EB1 overexpression enhanced AURKB kinase activity, and knockdown of EB1 with small interfering RNA impaired AURKB activity. EB1 protected AURKB from dephosphorylation/inactivation by protein phosphatase-2A by blocking binding of PP2A to AURKB. 

Gene Full Name: aurora kinase B

Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

Molecular Weight: 38 kDa

Gene ID: 20877

UniProt: O70126

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades