Survivin antibody (AA 1-140)

Catalog No. ABIN7972021

The Rabbit Polyclonal anti-Survivin antibody is suitable to detect Survivin in samples from Mouse. It has been validated for WB, IHC and FACS.

Quick Overview for Survivin antibody (AA 1-140) (ABIN7972021)

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Survivin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Survivin Antibody

Binding Specificity: AA 1-140

Purpose: Anti-Survivin/BIRC5 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Mouse Survivin shares 85%,91% amino acid (aa) sequence identity with human,rat Survivin,respectively.

Characteristics: Anti-Survivin/BIRC5 Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Survivin recombinant protein (Position: M1-A140). Mouse Survivin shares 85% and 91% amino acid (aa) sequence identity with human and rat Survivin, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mous Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Survivin

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Alternative Name: BIRC5

Background:

Background: Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that in humans encoded by the BIRC5 gene. Survivin is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. This gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions as inhibitor caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. The survivin protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.

Gene Full Name: baculoviral IAP repeat-containing 5

Sequence Similarities: Belongs to the IAP family.

Molecular Weight: 16 kDa

Gene ID: 11799

UniProt: O70201

Pathways: Apoptosis, Cell Division Cycle, Nuclear Hormone Receptor Binding