BID antibody (AA 1-195) (Biotin)

Catalog No. ABIN7972718

The Biotin-conjugated Mouse Monoclonal anti-BID antibody (Clone 10F4) (ABIN7972718) specifically detects BID in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for BID antibody (AA 1-195) (Biotin) (ABIN7972718)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BID antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 10F4

Product Details anti-BID Antibody (Biotin)

Binding Specificity: AA 1-195

Purpose: Anti-Bid Antibody (monoclonal, 10F4) Biotin Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human Bid shares 64%,61% amino acid (aa) sequences identity with mouse,rat Bid,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively.

Isotype: IgG2b

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID

Background:

Background: BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.

Gene Full Name: BH3 interacting domain death agonist

Gene ID: 637

UniProt: P55957

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity