CAMKK1 antibody (N-Term) (Biotin)

Catalog No. ABIN7974182

The Biotin-conjugated Rabbit Polyclonal anti-CAMKK1 antibody (ABIN7974182) specifically detects CAMKK1 in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CAMKK1 antibody (N-Term) (Biotin) (ABIN7974182)

Target: CAMKK1 (Calcium/calmodulin-Dependent Protein Kinase Kinase 1, alpha (CAMKK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMKK1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CAMKK1 Antibody (Biotin)

Binding Specificity: N-Term

Purpose: Anti-CaMKK/CAMKK1 Antibody Biotin Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related human sequence by three amino acids,and from the related rat sequence by two amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse CaMKK, different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CAMKK1

Target: CAMKK1 (Calcium/calmodulin-Dependent Protein Kinase Kinase 1, alpha (CAMKK1))

Alternative Name: CAMKK1

Background:

Background: CAMKK1, Calcium/calmodulin-dependent protein kinase kinase 1 is an enzyme that in humans is encoded by the CAMKK1 gene. The CAMKK1 gene is mapped to chromosome 17. The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca (2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene.Camkk1binds calmodulin and activated Camk4 with a 6-fold increase in total activity and a 100-fold increase in Camk4 Ca (2+)-independent activity. Camkk1 catalyzed a 10-fold increase in the total activity of Camk1 and had no effect on Camk2. Cotransfection of COS-7 cells with Camkk1 and Camk4 resulted in a 14-fold increase in CRE-binding protein-dependent transcription compared with Camk4 alone, suggesting that Camkk1 enhances Camk4-mediated transcriptional regulation.

Gene Full Name: calcium/calmodulin-dependent protein kinase kinase 1, alpha

Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Gene ID: 55984

UniProt: Q8VBY2

Pathways: AMPK Signaling