Calpain 2 antibody (AA 500-700)

Catalog No. ABIN7974357

This Mouse Monoclonal antibody specifically detects Calpain 2 in WB, IHC, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Monkey.

Quick Overview for Calpain 2 antibody (AA 500-700) (ABIN7974357)

Target: Calpain 2 (CAPN2)

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calpain 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Grade: Carrier-free

Clone: 8I6

Product Details anti-Calpain 2 Antibody

Binding Specificity: AA 500-700

Purpose: Anti-Calpain 2 CAPN2 Antibody (monoclonal, 8I6)

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: coli-derived human Calpain 2 recombinant protein (Position: R500-L700). Human Calpain 2 shares 93.5%,92.5% amino acid (aa) sequence identity with mouse,rat Calpain 2,respectively.

Characteristics: Anti-Calpain 2 CAPN2 Antibody.(monoclonal, 8I6). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Calpain 2 recombinant protein (Position: R500-L700). Human Calpain 2 shares 93.5% and 92.5% amino acid (aa) sequence identity with mouse and rat Calpain 2, respectively.

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Calpain 2

Target: Calpain 2 (CAPN2)

Alternative Name: CAPN2

Background:

Background: Calpain-2 catalytic subunit is a protein that in humans is encoded by the CAPN2 gene. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported.

Gene Full Name: calpain 2

Molecular Weight: 80 kDa

Gene ID: 824

UniProt: P17655