Calreticulin antibody (AA 333-365) (FITC)

Catalog No. ABIN7974462

This Rabbit Polyclonal antibody specifically detects Calreticulin in FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Calreticulin antibody (AA 333-365) (FITC) (ABIN7974462)

Target: Calreticulin (CALR)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calreticulin antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-Calreticulin Antibody (FITC)

Binding Specificity: AA 333-365

Purpose: Anti-Calreticulin/CALR Antibody FITC Conjugated

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human CALR shares 100% amino acid (aa) sequence identity with both mouse,rat CALR.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Calreticulin/CALR recombinant protein (Position: T333-Q365). Human CALR shares 100% amino acid (aa) sequence identity with both mouse and rat CALR.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: CALR

Background:

Background: Calreticulin also known as calregulin, CRP55, CaBP3, calsequestrin-like protein, and endoplasmic reticulum resident protein 60 (ERp60) is a protein that in humans is encoded by the CALR gene. It is mapped to 19p13.13. Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

Gene Full Name: calreticulin

Gene ID: 811

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response