CEBPA antibody (AA 138-221)

Catalog No. ABIN7976096

The Rabbit Polyclonal anti-CEBPA antibody (ABIN7976096) specifically detects CEBPA in WB, ELISA, FACS, ICC and IF. The antibody is reactive with Human samples.

Quick Overview for CEBPA antibody (AA 138-221) (ABIN7976096)

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-CEBPA Antibody

Binding Specificity: AA 138-221

Purpose: Anti-CEBP Alpha/CEBPA Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CEBP Alpha/CEBPA Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CEBP Alpha/CEBPA recombinant protein (Position: Y138-Q221).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CEBPA

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Alternative Name: CEBPA

Background:

Background: CEBPA, CCAAT/enhancer-binding protein alpha is a protein that in humans is encoded by the CEBPA gene. The CEBPA gene is intronless. Using human/hamster somatic cell hybrids containing restricted fragments of human chromosome 19, the CEBPA gene is mapped to chromosome 19q13.1, between the GPI and TGFB1 genes. The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis.

Gene Full Name: CCAAT enhancer binding protein alpha

Molecular Weight: 42 kDa

Gene ID: 1050

UniProt: P49715

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation