CDCA8 antibody (AA 72-280)

Catalog No. ABIN7977989

The Rabbit Polyclonal anti-CDCA8 antibody (ABIN7977989) specifically detects CDCA8 in WB, ELISA, FACS, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for CDCA8 antibody (AA 72-280) (ABIN7977989)

Target: CDCA8 (Cell Division Cycle Associated 8 (CDCA8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDCA8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-CDCA8 Antibody

Binding Specificity: AA 72-280

Purpose: Anti-CDCA8 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human CDCA8 shares 76%,77.8% amino acid (aa) sequence identity with mouse,rat CDCA8,respectively.

Characteristics: Anti-CDCA8 Antibody. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CDCA8 recombinant protein (Position: D72-K280). Human CDCA8 shares 76% and 77.8% amino acid (aa) sequence identity with mouse and rat CDCA8, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CDCA8

Target: CDCA8 (Cell Division Cycle Associated 8 (CDCA8))

Alternative Name: CDCA8

Background:

Background: Borealin is a protein that in humans is encoded by the CDCA8 gene. This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16.

Gene Full Name: cell division cycle associated 8

Molecular Weight: 35 kDa

Gene ID: 55143

UniProt: Q53HL2

Pathways: Cell Division Cycle, M Phase