CENPE antibody (AA 1390-2474)

Catalog No. ABIN7978066

The Rabbit Polyclonal anti-CENPE antibody is suitable to detect CENPE in samples from Rat and Mouse. It has been validated for WB and ELISA.

Quick Overview for CENPE antibody (AA 1390-2474) (ABIN7978066)

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPE antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Carrier-free

Product Details anti-CENPE Antibody

Binding Specificity: AA 1390-2474

Purpose: Anti-Cenpe Antibody

Predicted Reactivity: Mouse Cenpe shares 49.9% amino acid (aa) sequence identity with human Cenpe,respectively.

Characteristics: Anti-Cenpe Antibody. Tested in WB, ELISA applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Cenpe recombinant protein (Position: Q1390-Q2474). Mouse Cenpe shares 49.9% amino acid (aa) sequence identity with human Cenpe, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CENPE

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Alternative Name: Cenpe

Background:

Background: Centromere-associated protein E is a protein that in humans is encoded by the CENPE gene. Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms.

Gene Full Name: centromere protein E

Molecular Weight: 300 kDa

Gene ID: 229841

UniProt: Q6RT24

Pathways: M Phase, Maintenance of Protein Location