CCT4 antibody (AA 452-539)

Catalog No. ABIN7978286

The Rabbit Polyclonal anti-CCT4 antibody (ABIN7978286) specifically detects CCT4 in WB, IHC, IF, FACS, IP, ICC and IHC (fro). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CCT4 antibody (AA 452-539) (ABIN7978286)

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: Carrier-free

Product Details anti-CCT4 Antibody

Binding Specificity: AA 452-539

Purpose: Anti-TCP1 delta/CCT4 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: coli-derived human CCT4 recombinant protein (Position: R452-R539). Human CCT4 shares 97.7% amino acid (aa) sequence identity with both mouse,rat CCT4.

Characteristics: Anti-TCP1 delta/CCT4 Antibody. Tested in Flow Cytometry, IP, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CCT4 recombinant protein (Position: R452-R539). Human CCT4 shares 97.7% amino acid (aa) sequence identity with both mouse and rat CCT4.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human, Mouse, Rat Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CCT4

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Alternative Name: CCT4

Background:

Background: T-complex protein 1 subunit delta is a protein that in humans is encoded by the CCT4 gene. This gene is mapped to 2p15. The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha-transducin.

Gene Full Name: chaperonin containing TCP1 subunit 4

Molecular Weight: 58 kDa

Gene ID: 10575

UniProt: P50991

Pathways: Embryonic Body Morphogenesis