Prothrombin antibody (AA 97-124)

Catalog No. ABIN7980697

The Mouse Monoclonal anti-Prothrombin antibody is suitable to detect Prothrombin in samples from Human, Rat and Mouse. It has been validated for WB.

Quick Overview for Prothrombin antibody (AA 97-124) (ABIN7980697)

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prothrombin antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Clone: 5B6G1

Product Details anti-Prothrombin Antibody

Binding Specificity: AA 97-124

Purpose: Anti-Prothrombin Antibody (monoclonal, 5B6G1)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Prothrombin Antibody.(monoclonal, 5B6G1)-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Prothrombin recombinant protein (Position: Y97-R124).

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Prothrombin

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Alternative Name: F2

Background:

Background: F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1). Thrombin also inhibits coagulation by activating protein C.

Gene Full Name: coagulation factor II, thrombin

Molecular Weight: 90 kDa

Gene ID: 2147

UniProt: P00734

Pathways: Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling