C3 antibody (AA 1277-1538)

Catalog No. ABIN7981381

The Rabbit Polyclonal anti-C3 antibody is suitable to detect C3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for C3 antibody (AA 1277-1538) (ABIN7981381)

Target: C3 (Complement Component 3 (C3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-C3 Antibody

Binding Specificity: AA 1277-1538

Purpose: Anti-C3 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human C3 shares 73.7 %,76.3% amino acid (aa) sequence identity with mouse,rat C3,respectively.

Characteristics: Anti-C3 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human C3 recombinant protein (Position: Q1277-E1538). Human C3 shares 73.7 % and 76.3% amino acid (aa) sequence identity with mouse and rat C3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: C3

Background:

Background: Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.

Gene Full Name: complement C3

Molecular Weight: 128 kDa, 187 kDa

Gene ID: 718

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling