COPS5 antibody (AA 8-334)

Catalog No. ABIN7981678

The Mouse Monoclonal anti-COPS5 antibody is suitable to detect COPS5 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, ICC and FACS.

Quick Overview for COPS5 antibody (AA 8-334) (ABIN7981678)

Target: COPS5 (COP9 Constitutive Photomorphogenic Homolog Subunit 5 (Arabidopsis) (COPS5))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This COPS5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Grade: Carrier-free

Clone: 4G9

Product Details anti-COPS5 Antibody

Binding Specificity: AA 8-334

Purpose: Anti-JAB1 Antibody (monoclonal, 4G9)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human JAB1 shares 99.4% amino acid (aa) sequence identity with mouse JAB1.

Characteristics: Anti-JAB1 Antibody.(monoclonal, 4G9)-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human JAB1 recombinant protein (Position: M8-S334). Human JAB1 shares 99.4% amino acid (aa) sequence identity with mouse JAB1.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for COPS5

Target: COPS5 (COP9 Constitutive Photomorphogenic Homolog Subunit 5 (Arabidopsis) (COPS5))

Alternative Name: COPS5

Background:

Background: COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis), also known as COPS5 or JAB1, is a gene conserved from humans to Saccharomyces cerevisiae. It is a member of the MOV34 family. COPS5 is mapped to 8q13.1. The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. COPS5 can interact with the cytoplasmic domain of the beta-2 subunit of the alpha-L/beta-2 integrin LFA1, and it is the only protein demonstrated to interact with MIF. COPS5, VHL, and TRC8 proteins appear to be linked both physically and functionally, and all 3 may participate in the development of kidney cancer. In addition to that, COPS5 is an essential cofactor for the apoptotic function of E2F1.

Gene Full Name: COP9 signalosome subunit 5

Molecular Weight: 37 kDa

Gene ID: 10987

UniProt: Q92905

Pathways: Cell Division Cycle