Cullin 1 antibody (AA 457-770)

Catalog No. ABIN7981987

The Rabbit Polyclonal anti-Cullin 1 antibody is suitable to detect Cullin 1 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for Cullin 1 antibody (AA 457-770) (ABIN7981987)

Target: Cullin 1 (CUL1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-Cullin 1 Antibody

Binding Specificity: AA 457-770

Purpose: Anti-Cullin 1/CUL1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Cullin 1 shares 100% amino acid (aa) sequence identity with mouse Cullin 1.

Characteristics: Anti-Cullin 1/CUL1 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cullin 1 recombinant protein (Position: E457-D770). Human Cullin 1 shares 100% amino acid (aa) sequence identity with mouse Cullin 1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cullin 1

Target: Cullin 1 (CUL1)

Alternative Name: CUL1

Background:

Background: Cullin 1, also known as CUL1, is a human protein and gene from cullin family. This protein plays an important role in protein degradation and protein ubiquitination. This is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Human cullin 1, but not the other closely related cullins 2, 3, 4A, and 5, selectively interacts with human SKP1.

Gene Full Name: cullin 1

Sequence Similarities: Belongs to the CCN family.

Molecular Weight: 90 kDa

UniProt: Q13616

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases, Regulation of Cell Size