CYBB antibody (AA 416-500)

Catalog No. ABIN7982802

This Rabbit Polyclonal antibody specifically detects CYBB in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CYBB antibody (AA 416-500) (ABIN7982802)

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYBB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Carrier-free

Product Details anti-CYBB Antibody

Binding Specificity: AA 416-500

Purpose: Anti-NOX2/gp91phox/CYBB Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NOX2/gp91phox/CYBB Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human NOX2/gp91phox recombinant protein (Position: F416-D500).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Alternative Name: CYBB

Background:

Background: NOX2 (NADPH OXIDASE 2), also called CYBB (CYTOCHROME b (-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein. NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling).

Gene Full Name: cytochrome b-245 beta chain

Molecular Weight: 65 kDa

Gene ID: 1536

UniProt: P04839