DDB2 antibody (AA 1-115)

Catalog No. ABIN7983763

The Rabbit Polyclonal anti-DDB2 antibody is suitable to detect DDB2 in samples from Human. It has been validated for WB, FACS, ICC and IF.

Quick Overview for DDB2 antibody (AA 1-115) (ABIN7983763)

Target: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDB2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-DDB2 Antibody

Binding Specificity: AA 1-115

Purpose: Anti-DDB2 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human DDB2 shares 58.3% amino acid (aa) sequence identity with mouse DDB2.

Characteristics: Anti-DDB2 Antibody. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DDB2 recombinant protein (Position: M1-T115). Human DDB2 shares 58.3% amino acid (aa) sequence identity with mouse DDB2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DDB2

Target: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Alternative Name: DDB2

Background:

Background: DNA damage-binding protein 2 is a protein that in humans is encoded by the DDB2 gene. This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. And this subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: damage specific DNA binding protein 2

Molecular Weight: 48 kDa

Gene ID: 1643

UniProt: Q92466

Pathways: DNA Damage Repair