DBF4 antibody (AA 295-665)

Catalog No. ABIN7983808

The Rabbit Polyclonal anti-DBF4 antibody is suitable to detect DBF4 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF, FACS and ICC.

Quick Overview for DBF4 antibody (AA 295-665) (ABIN7983808)

Target: DBF4 (DBF4 Homolog (DBF4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-DBF4 Antibody

Binding Specificity: AA 295-665

Purpose: Anti-DBF4 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DBF4 Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DBF4 recombinant protein (Position:Y295-S665).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DBF4

Target: DBF4 (DBF4 Homolog (DBF4))

Alternative Name: DBF4

Background:

Background: Using both gain- and loss-of-function experiments, it was found that Xenopus Dbf4 inhibited canonical Wnt signaling. Depletion of endogenous Dbf4 did not disturb gastrulation movements or early organizer genes, but resulted in embryos with morphologically defective heart and eyes and suppressed cardiac markers. The function of Dbf4 in heart development appeared to be independent of its role in the cell cycle. Dbf4 interacted physically and functionally with Frodo, a context-dependent regulator of Wnt signaling.

Gene Full Name: DBF4-CDC7 kinase regulatory subunit

Molecular Weight: 80 kDa

Gene ID: 10926

UniProt: Q9UBU7

Pathways: Mitotic G1-G1/S Phases, DNA Replication