DDX27 antibody (AA 41-744)

Catalog No. ABIN7984087

The Rabbit Polyclonal anti-DDX27 antibody is suitable to detect DDX27 in samples from Human, Mouse and Rat. It has been validated for ELISA, WB, IF, IP and ICC.

Quick Overview for DDX27 antibody (AA 41-744) (ABIN7984087)

Target: DDX27 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 27 (DDX27))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX27 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-DDX27 Antibody

Binding Specificity: AA 41-744

Purpose: Anti-DDX27 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DDX27 Antibody. Tested in WB, ICC, IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DDX27 recombinant protein (Position: Q41-L744).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DDX27

Target: DDX27 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 27 (DDX27))

Alternative Name: DDX27

Background:

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein involved in the processing of 5.8S and 28S ribosomal RNAs. More specifically, the encoded protein localizes to the nucleolus, where it interacts with the PeBoW complex to ensure proper 3' end formation of 47S rRNA.

Gene Full Name: DEAD-box helicase 27

Molecular Weight: 87 kDa

Gene ID: 55661

UniProt: Q96GQ7