DDX4 antibody (Middle Region) (HRP)

Catalog No. ABIN7984347

This Rabbit Polyclonal antibody specifically detects DDX4 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DDX4 antibody (Middle Region) (HRP) (ABIN7984347)

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX4 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-DDX4 Antibody (HRP)

Binding Specificity: Middle Region

Purpose: Anti-DDX4/MVH Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the the related rat,mouse sequences.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human DDX4, identical to the the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DDX4

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Alternative Name: DDX4

Background:

Background: DDX4 (DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al. (2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog (Ddx4) resides (Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al. (2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.

Gene Full Name: DEAD-box helicase 4

Sequence Similarities: Belongs to the DEAD box helicase family. DDX4/VASA subfamily.

Gene ID: 54514

UniProt: Q9NQI0