TdT antibody (AA 316-509) (Fluoro550)

Catalog No. ABIN7984879

The Rabbit Polyclonal anti-TdT antibody is suitable to detect TdT in samples from Human. It has been validated for FACS.

Quick Overview for TdT antibody (AA 316-509) (Fluoro550) (ABIN7984879)

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TdT antibody is conjugated to Fluoro550

Application: Flow Cytometry (FACS)

Product Details anti-TdT Antibody (Fluoro550)

Binding Specificity: AA 316-509

Purpose: Anti-TdT/DNTT Antibody Fluoro550 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for TdT

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Alternative Name: DNTT

Background:

Background: Terminal Deoxynucleotidyl Transferase, also known as TDT and terminal transferase, is a unique DNA polymerase without template ion catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TDT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TDT may be responsible for inserting nucleotides (N regions) at the V (H)-D and D-J (H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TDT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.

Gene Full Name: DNA nucleotidylexotransferase

Sequence Similarities: Belongs to the DNA polymerase type-X family.

Gene ID: 1791

UniProt: P04053

Pathways: DNA Damage Repair