DPYD antibody (N-Term)

Catalog No. ABIN7985422

The Rabbit Polyclonal anti-DPYD antibody is suitable to detect DPYD in samples from Human, Mouse and Rat. It has been validated for WB and FACS.

Quick Overview for DPYD antibody (N-Term) (ABIN7985422)

Target: DPYD (Dihydropyrimidine Dehydrogenase (DPYD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPYD antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-DPYD Antibody

Binding Specificity: N-Term

Purpose: Anti-DPYD Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat,mouse sequences by one amino acid.

Characteristics: Anti-DPYD Antibody. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human DPYD, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DPYD

Target: DPYD (Dihydropyrimidine Dehydrogenase (DPYD))

Alternative Name: DPYD

Background:

Background: DPYD (Dihydropyrimidine Dehydrogenase), also called DPD, is an enzyme that in humans is encoded by the DPYD gene. The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. The structure of the DPYD gene contains 23 exons spanning about 950 kb. Using somatic cell hybrid strategies, the DPYD gene is mapped to the centromeric region of chromosome 1 between 1p22 and 1q21. By fluorescence in situ hybridization, the DPYD gene is mapped to 1p22. The highest level of DPD was found in monocytes followed by that in lymphocytes, granulocytes, and platelets, whereas no significant activity of DPD could be detected in erythrocytes. The activity of DPD in peripheral blood mononuclear cells was intermediate between that observed in monocytes and lymphocytes. By cDNA microarray, Western blot analysis, and luciferase reporter assay, the transcription factor LSF was identified as a positive regulator of DPYD.

Gene Full Name: dihydropyrimidine dehydrogenase

Sequence Similarities: Belongs to the dihydropyrimidine dehydrogenase family.

Molecular Weight: 11 kDa

Gene ID: 1806

UniProt: Q12882

Pathways: Ribonucleoside Biosynthetic Process