RAD51 antibody (AA 1-258) (HRP)

Catalog No. ABIN7985968

The Rabbit Polyclonal anti-RAD51 antibody is suitable to detect RAD51 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for RAD51 antibody (AA 1-258) (HRP) (ABIN7985968)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD51 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-RAD51 Antibody (HRP)

Binding Specificity: AA 1-258

Purpose: Anti-Rad51 Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Rad51 shares 98% amino acid (aa) sequence identity with mouse Rad51.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Rad51 recombinant protein (Position: M1-E258). Human Rad51 shares 98% amino acid (aa) sequence identity with mouse Rad51.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name: RAD51

Background:

Background: DNA repair protein RAD51 homolog 1, also known as RAD51A, is a human gene. The Rad51 gene, HsRAD51, is a homolog of RecA of Escherichia coli and functions in recombination and DNA repair. BRCA1 and BRCA2 proteins form a complex with Rad51, and these genes are thought to participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. RAD51 is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.

Gene Full Name: RAD51 recombinase

Sequence Similarities: Belongs to the RecA family. RAD51 subfamily.

Gene ID: 5888

UniProt: Q06609

Pathways: DNA Damage Repair