DNAJA1 antibody (C-Term)

Catalog No. ABIN7986049

The Rabbit Polyclonal anti-DNAJA1 antibody is suitable to detect DNAJA1 in samples from Human, Mouse and Rat. It has been validated for WB, IF and ICC.

Quick Overview for DNAJA1 antibody (C-Term) (ABIN7986049)

Target: DNAJA1 (DnaJ (Hsp40) Homolog, Subfamily A, Member 1 (DNAJA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-DNAJA1 Antibody

Binding Specificity: C-Term

Purpose: Anti-HDJ2/DNAJA1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Characteristics: Anti-HDJ2/DNAJA1 Antibody. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HDJ2, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DNAJA1

Target: DNAJA1 (DnaJ (Hsp40) Homolog, Subfamily A, Member 1 (DNAJA1))

Alternative Name: DNAJA1

Background:

Background: DNAJA1 (DnaJ homolog subfamily A member 1), also called DJA1, HSDJ, HSPF4, is a protein that in humans is encoded by the DNAJA1 gene. The deduced 397-amino acid DNAJA1 protein is 32 % identical to E. coli DnaJ, with the highest identity in the N-terminal region. Among the known DNAJ homologs in S. cerevisiae, DNAJA1 is most identical to YDJ1, which may be involved in the transport of certain proteins into the mitochondria and endoplasmic reticulum. Dja1 is expressed in mouse testis and prostate by immunohistochemical analysis and Western blot analysis. Transplantation experiments with fluorescence-tagged spermatogonia into Dja1 -/- mice revealed a primary defect of Sertoli cells in maintaining spermiogenesis at steps 8 and 9.

Gene Full Name: DnaJ heat shock protein family (Hsp40) member A1

Sequence Similarities: Contains 1 CR-type zinc finger.

Molecular Weight: 45 kDa

Gene ID: 3301

UniProt: P31689

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway