E2F6 antibody (Middle Region) (Fluoro488)

Catalog No. ABIN7986824

The Fluoro488-conjugated Rabbit Polyclonal anti-E2F6 antibody (ABIN7986824) specifically detects E2F6 in FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for E2F6 antibody (Middle Region) (Fluoro488) (ABIN7986824)

Target: E2F6 (E2F Transcription Factor 6 (E2F6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F6 antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Product Details anti-E2F6 Antibody (Fluoro488)

Binding Specificity: Middle Region

Purpose: Anti-Transcription factor E2F6 E2F6 Antibody Fluoro488 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse,rat sequences by one amino acid.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human E2F6, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for E2F6

Target: E2F6 (E2F Transcription Factor 6 (E2F6))

Alternative Name: E2F6

Background:

Background: E2F6 (E2F transcription factor 6) also known as E2F-6. Northern blot analysis revealed that E2F6 is expressed as 2 mRNAs, 2.5 and 3.5 kb, in all human tissues and cell lines tested. The predicted human and mouse E2F6 protein sequences are 92% identical. The DNA-binding and dimerization domains of E2F6 are highly related to those of other E2F family members, but this protein lacks the sequences necessary for either transcriptional activation or binding to RB1, p107, or p130. E2F6 can act to repress the transcription of E2F-responsive genes by countering the activity of other E2F complexes. E2F6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. E2F6 is found in a multimeric protein complex that contains MGA and MAX, and thus the complex can bind not only to the E2F binding site but also to MYC- and Brachyury- binding sites. The E2F6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells.

Gene Full Name: E2F transcription factor 6

Sequence Similarities: Belongs to the E2F/DP family.

Gene ID: 1876

UniProt: O75461