E2F5 antibody (AA 121-346) (Biotin)

Catalog No. ABIN7986899

The Biotin-conjugated Rabbit Polyclonal anti-E2F5 antibody (ABIN7986899) specifically detects E2F5 in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for E2F5 antibody (AA 121-346) (Biotin) (ABIN7986899)

Target: E2F5

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F5 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-E2F5 Antibody (Biotin)

Binding Specificity: AA 121-346

Purpose: Anti-E2F5 Antibody Biotin Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human E2F5 recombinant protein (Position: C121-Y346).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for E2F5

Target: E2F5

Alternative Name: E2F5

Background:

Background: Transcription factor E2F5 is a protein that in humans is encoded by the E2F5 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.

Gene Full Name: E2F transcription factor 5

Gene ID: 1875

UniProt: Q15329