SIAH1 antibody (Middle Region)

Catalog No. ABIN7986954

This Rabbit Polyclonal antibody specifically detects SIAH1 in WB, IHC, IF, FACS and ICC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for SIAH1 antibody (Middle Region) (ABIN7986954)

Target: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIAH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-SIAH1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-SIAH1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SIAH1 Antibody. Tested in WB, IHC, ICC, IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SIAH1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SIAH1

Target: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Alternative Name: SIAH1

Background:

Background: This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Gene Full Name: siah E3 ubiquitin protein ligase 1

Molecular Weight: 65 kDa

Gene ID: 6477

UniProt: Q8IUQ4