EPAS1 antibody (Middle Region) (PE)

Catalog No. ABIN7987705

The Rabbit Polyclonal anti-EPAS1 antibody is suitable to detect EPAS1 in samples from Human, Rat and Mouse. It has been validated for FACS.

Quick Overview for EPAS1 antibody (Middle Region) (PE) (ABIN7987705)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPAS1 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Product Details anti-EPAS1 Antibody (PE)

Binding Specificity: Middle Region

Purpose: Anti-HIF-2-alpha/EPAS1 Antibody PE Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related mouse,rat sequence.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human HIF-2-alpha, identical to the related mouse and rat sequence.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: EPAS1

Background:

Background: HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88 % identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event1. Moreover, the predicted protein shares 48 % sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia2. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined3. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration4.

Gene Full Name: endothelial PAS domain protein 1

Sequence Similarities: Contains 1 bHLH (basic helix-loop-helix) domain.

Gene ID: 29452

UniProt: Q9JHS1

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect