EZH2 antibody (AA 22-345)

Catalog No. ABIN7987807

The Rabbit Polyclonal anti-EZH2 antibody is suitable to detect EZH2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, FACS and IP.

Quick Overview for EZH2 antibody (AA 22-345) (ABIN7987807)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EZH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Grade: Carrier-free

Product Details anti-EZH2 Antibody

Binding Specificity: AA 22-345

Purpose: Anti-KMT6/EZH2 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-KMT6/EZH2 Antibody. Tested in ELISA, Flow Cytometry, IP, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human KMT6/EZH2 recombinant protein (Position: E22-T345).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunoprecipitation, 0.5-2 μg/mL Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EZH2

Background:

Background: Enhancer of zeste homolog 2 (EZH2) is a histone-lysine N-methyltransferase enzyme encoded by EZH2 gene. It is mapped to 7q36.1. This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.

Gene Full Name: enhancer of zeste 2 polycomb repressive complex 2 subunit

Molecular Weight: 98 kDa

Gene ID: 2146

UniProt: Q15910

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation