EIF2A antibody (AA 24-585)

Catalog No. ABIN7988755

The Rabbit Polyclonal anti-EIF2A antibody is suitable to detect EIF2A in samples from Human and Rat. It has been validated for WB, IHC, FACS and ELISA.

Quick Overview for EIF2A antibody (AA 24-585) (ABIN7988755)

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Grade: Carrier-free

Product Details anti-EIF2A Antibody

Binding Specificity: AA 24-585

Purpose: Anti-EIF2A Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human EIF2A shares 92.5% amino acid (aa) sequence identity with mouse EIF2A.

Characteristics: Anti-EIF2A Antibody. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EIF2A recombinant protein (Position: H24-I585). Human EIF2A shares 92.5% amino acid (aa) sequence identity with mouse EIF2A.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: EIF2A

Background:

Background: This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Gene Full Name: eukaryotic translation initiation factor 2A

Molecular Weight: 65 kDa

Gene ID: 83939

UniProt: Q9BY44

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly