Fibrillarin antibody (AA 77-138) (HRP)

Catalog No. ABIN7990285

The Rabbit Polyclonal anti-Fibrillarin antibody is suitable to detect Fibrillarin in samples from Human and Monkey. It has been validated for WB, IHC and ELISA.

Quick Overview for Fibrillarin antibody (AA 77-138) (HRP) (ABIN7990285)

Target: Fibrillarin (FBL)

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibrillarin antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Fibrillarin Antibody (HRP)

Binding Specificity: AA 77-138

Purpose: Anti-Fibrillarin/FBL Antibody HRP Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Fibrillarin/FBL recombinant protein (Position: K77-N138).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Fibrillarin

Target: Fibrillarin (FBL)

Alternative Name: FBL

Background:

Background: rRNA 2'-O-methyltransferase fibrillarin is an enzyme that in humans is encoded by the FBL gene. This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8 % of humans with the autoimmune disease scleroderma recognize fibrillarin.

Gene Full Name: fibrillarin rRNA 2'-O-methyltransferase

Gene ID: 2091

UniProt: P22087

Pathways: Ribonucleoside Biosynthetic Process