Fibrillarin antibody (AA 77-138)

Catalog No. ABIN7990289

The Rabbit Polyclonal anti-Fibrillarin antibody (ABIN7990289) specifically detects Fibrillarin in WB, IF, ELISA, ICC and FACS. The antibody is reactive with Human and Monkey samples.

Quick Overview for Fibrillarin antibody (AA 77-138) (ABIN7990289)

Target: Fibrillarin (FBL)

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibrillarin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Fibrillarin Antibody

Binding Specificity: AA 77-138

Purpose: Anti-Fibrillarin/FBL Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Fibrillarin/FBL Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Fibrillarin/FBL recombinant protein (Position: K77-N138).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Monkey Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Fibrillarin

Target: Fibrillarin (FBL)

Alternative Name: FBL

Background:

Background: rRNA 2'-O-methyltransferase fibrillarin is an enzyme that in humans is encoded by the FBL gene. This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8 % of humans with the autoimmune disease scleroderma recognize fibrillarin.

Gene Full Name: fibrillarin rRNA 2'-O-methyltransferase

Molecular Weight: 37 kDa

Gene ID: 2091

UniProt: P22087

Pathways: Ribonucleoside Biosynthetic Process