Geminin antibody (AA 21-196)

Catalog No. ABIN7993069

The Rabbit Polyclonal anti-Geminin antibody is suitable to detect Geminin in samples from Human and Mouse. It has been validated for WB, IHC, IF, ELISA, ICC and FACS.

Quick Overview for Geminin antibody (AA 21-196) (ABIN7993069)

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Geminin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Geminin Antibody

Binding Specificity: AA 21-196

Purpose: Anti-Geminin/GMNN Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Geminin/GMNN Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Geminin/GMNN recombinant protein (Position: V21-E196).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Geminin

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Alternative Name: GMNN

Background:

Background: Geminin, DNA replication inhibitor, also known as GMNN, is a protein in humans encoded by the GMNN gene. This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16.

Gene Full Name: geminin DNA replication inhibitor

Molecular Weight: 25 kDa

Gene ID: 51053

UniProt: O75496

Pathways: EGFR Signaling Pathway, DNA Replication