GLI3 antibody (N-Term) (Fluoro594)

Catalog No. ABIN7993261

The Rabbit Polyclonal anti-GLI3 antibody is suitable to detect GLI3 in samples from Human, Mouse and Rat. It has been validated for FACS.

Quick Overview for GLI3 antibody (N-Term) (Fluoro594) (ABIN7993261)

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI3 antibody is conjugated to Fluoro594

Application: Flow Cytometry (FACS)

Product Details anti-GLI3 Antibody (Fluoro594)

Binding Specificity: N-Term

Purpose: Anti-Transcriptional activator GLI3 Gli3 Antibody Fluoro594 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat sequence by two amino acids,and from the related mouse sequence by one amino acid.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3, different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Alternative Name: GLI3

Background:

Background: GLI3 (Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila. The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus (ci) gene product (Ci), which regulates the Patched (pct), gooseberry (gsb), and decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.

Gene Full Name: GLI family zinc finger 3

Sequence Similarities: Belongs to the GLI C2H2-type zinc-finger protein family.

Gene ID: 2737

UniProt: P10071

Pathways: Hedgehog Signaling