Grancalcin antibody (AA 42-217)

Catalog No. ABIN7995019

The Rabbit Polyclonal anti-Grancalcin antibody is suitable to detect Grancalcin in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, ELISA, FACS and ICC.

Quick Overview for Grancalcin antibody (AA 42-217) (ABIN7995019)

Target: Grancalcin (GCA) (Grancalcin, EF-Hand Calcium Binding Protein (GCA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Grancalcin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-Grancalcin Antibody

Binding Specificity: AA 42-217

Purpose: Anti-Grancalcin/GCA Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Grancalcin/GCA Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Grancalcin/GCA recombinant protein (Position: A42-I217).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL Immunocytochemistry/Immunofluorescence, 5 μg/mL Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Grancalcin

Target: Grancalcin (GCA) (Grancalcin, EF-Hand Calcium Binding Protein (GCA))

Alternative Name: GCA

Background:

Background: Grancalcin is a protein that in humans is encoded by the GCA gene. This gene is mapped to 2q24.2. This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction, with magnesium alone, it partitions with the granule fraction, and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.

Gene Full Name: grancalcin

Molecular Weight: 25 kDa

Gene ID: 25801

UniProt: P28676