HCK antibody (AA 14-501)

Catalog No. ABIN7996967

The Rabbit Polyclonal anti-HCK antibody (ABIN7996967) specifically detects HCK in WB, ELISA and FACS. The antibody is reactive with Human and Rat samples.

Quick Overview for HCK antibody (AA 14-501) (ABIN7996967)

Target: HCK (Hemopoietic Cell Kinase (HCK))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-HCK Antibody

Binding Specificity: AA 14-501

Purpose: Anti-HCK Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human HCK shares 89.2%,88.7% amino acid (aa) sequence identity with mouse,rat HCK,respectively.

Characteristics: Anti-HCK Antibody. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HCK recombinant protein (Position: R14-R501). Human HCK shares 89.2% and 88.7% amino acid (aa) sequence identity with mouse and rat HCK, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HCK

Target: HCK (Hemopoietic Cell Kinase (HCK))

Alternative Name: HCK

Background:

Background: The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon.

Gene Full Name: HCK proto-oncogene, Src family tyrosine kinase

Molecular Weight: 60 kDa

Gene ID: 3055

UniProt: P08631

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling