HNF4A antibody (AA 164-474) (APC)

Catalog No. ABIN7997195

The APC-conjugated Mouse Monoclonal anti-HNF4A antibody (Clone 6C8E9) (ABIN7997195) specifically detects HNF4A in FACS. The antibody is reactive with Human and Rat samples.

Quick Overview for HNF4A antibody (AA 164-474) (APC) (ABIN7997195)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNF4A antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: 6C8E9

Product Details anti-HNF4A Antibody (APC)

Binding Specificity: AA 164-474

Purpose: Anti-HNF-4-alpha Antibody (monoclonal, 6C8E9) APC Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human HNF-4-alpha shares 95%,96% amino acid (aa) sequence identity with mouse,rat HNF-4-alpha,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HNF-4-alpha recombinant protein (Position: Q164-I474). Human HNF-4-alpha shares 95% and 96% amino acid (aa) sequence identity with mouse and rat HNF-4-alpha, respectively.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4A

Background:

Background: Hepatocyte nuclear factor 4 alpha (HNF4A), also known as NR2A1, is a nuclear receptor that in humans is encoded by the HNF4A gene. It is mapped to 20q13.12. HNF4A is a nuclear transcription factor that binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene plays a role in development of the liver, kidney, and intestines. HNF4A is required for the PXR and CAR-mediated transcriptional activation of CYP3A4. This gene also plays a pivotal role in the expression and synthesis of SHBG, an important glycoprotein made primarily in the liver, which in addition to lowering insulin-resistance also serves in reducing levels of free Oestrogen as-well as prolonging the half-life of Testosterone.

Gene Full Name: hepatocyte nuclear factor 4 alpha

Gene ID: 3172

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process