HNF4A antibody (AA 164-474)

Catalog No. ABIN7997198

The Mouse Monoclonal anti-HNF4A antibody (Clone 6C8E9) (ABIN7997198) specifically detects HNF4A in WB and IHC. The antibody is reactive with Human and Rat samples.

Quick Overview for HNF4A antibody (AA 164-474) (ABIN7997198)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Clone: 6C8E9

Product Details anti-HNF4A Antibody

Binding Specificity: AA 164-474

Purpose: Anti-HNF-4-alpha Antibody (monoclonal, 6C8E9)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human HNF-4-alpha shares 95%,96% amino acid (aa) sequence identity with mouse,rat HNF-4-alpha,respectively.

Characteristics: Anti-HNF-4-alpha Antibody.(monoclonal, 6C8E9)-2. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HNF-4-alpha recombinant protein (Position: Q164-I474). Human HNF-4-alpha shares 95% and 96% amino acid (aa) sequence identity with mouse and rat HNF-4-alpha, respectively.

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4A

Background:

Background: Hepatocyte nuclear factor 4 alpha (HNF4A), also known as NR2A1, is a nuclear receptor that in humans is encoded by the HNF4A gene. It is mapped to 20q13.12. HNF4A is a nuclear transcription factor that binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene plays a role in development of the liver, kidney, and intestines. HNF4A is required for the PXR and CAR-mediated transcriptional activation of CYP3A4. This gene also plays a pivotal role in the expression and synthesis of SHBG, an important glycoprotein made primarily in the liver, which in addition to lowering insulin-resistance also serves in reducing levels of free Oestrogen as-well as prolonging the half-life of Testosterone.

Gene Full Name: hepatocyte nuclear factor 4 alpha

Molecular Weight: 53 kDa

Gene ID: 3172

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process