HSD17B1 antibody (N-Term) (Fluoro594)

Catalog No. ABIN7998756

This Rabbit Polyclonal antibody specifically detects HSD17B1 in FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for HSD17B1 antibody (N-Term) (Fluoro594) (ABIN7998756)

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B1 antibody is conjugated to Fluoro594

Application: Flow Cytometry (FACS)

Product Details anti-HSD17B1 Antibody (Fluoro594)

Binding Specificity: N-Term

Purpose: Anti-HSD17B1 Antibody Fluoro594 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat,mouse sequences by one amino acid.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HSD17B1, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for HSD17B1

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Alternative Name: HSD17B1

Background:

Background: Estradiol 17-beta-dehydrogenase 1 is an enzyme that in humans is encoded by the HSD17B1 gene. This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants.

Gene Full Name: hydroxysteroid 17-beta dehydrogenase 1

Sequence Similarities: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Gene ID: 3292

UniProt: P14061

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis